Mushroom Guru (Pty) Ltd.

Name: Glass Beakers (2 sizes)
SKU: P-052
Price: 65 ZAR
Availability: InStock

Mushroom Guru provides a one-stop-mushroom-cultivation shop. Browse our SHOP for spawn and supplies along with our advanced technology Reishi products.

Glass Beakers (2 sizes)

SKU P-052

R65.00

In stock

Quantity:

Product Details

A Glass Beaker is used in making an agar nutrient mixture that is poured into a Petri Dish and allowed to set. We call this a Nutrient Agar Plate (MEA) and it is used for transfers and cloning of mycelium.

Agar Agar Powder is an ingredient used in the preparation of MEA and PDA nutrient plates. It is a gelatinous substance obtained from seaweed and used in biological culture media and as a thickener in food.

MEA Ingredients:

11g Agar Agar Powder

7-10g Barley Malt Powder

500ml boiling water

PDA Ingredients:

11g Agar Agar Powder

1-3g Dextrose Powder

500ml hot potato broth

Method:

Mix all the ingredients very well and until there are no lumps.
Pour the mixture into a Glass Beaker and cover with Heavy Duty Foil and press down around the beaker (the foil is a splash cover).
Place the Glass Beaker in a Pressure Cooker and get it to operating temperature and pressure.
From this point, cook for 45 minutes.
Remove Glass Beaker from the Pressure Cooker and wait for the temperature of the mixture to drop to 40°C.
Pour into the Petri Dishes
Allow the mixture to cool, then seal with Parafilm
This recipe makes 40x65mm plates

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